Cell Culture Chamber
The QV600 chamber has been designed for surface cultures, such as skin, respiratory epithelium or whole corneas. Additionally, the use of transwells to create a double cavity chamber is ideal for the study of membranes and barrier models.
Is the QV600 system applicable to your project? Contact us to find out!
QV600
The QV600 provides a constant medium surface, positioned to allow the membrane on commercial inserts to sit at this height and provide cell cultures with a continuous perfusion of media as well as exposure to a controlled gaseous environment.
The QV600 chamber can also be fitted with an alternative insert to create a double cavity chamber ideal for the study of membranes and barrier models.
KEY BENEFITS
Users of the QV600 can expect to profit from a number of key benefits associate with the QV600 cell culture chambers:
- Continuous flow of medium across cell cultures
- Consistent ALI level within chambers – no risk of cells drying due to evaporation
- Ports for control of gas within culture
- Simple fluid level control
- Sized for commercially available inserts
SPECIFICATION
| Chamber width | 15 mm internal |
| Chamber depth | 33 mm, minimum depth 12 mm |
| Materials | Chamber: PDMS Tubing: Tygon Luers and reservoir bottle: Polypropylene |
| Overall dimensions | 23 mm height x 37 mm diameter |
| Diameter of tubing | Inlet: 1/16” ID Outlet: 3/32” ID |
| Volume of chamber | 2 ml running as ALI, 2 x 2 ml with barrier insert |
QV600 EVIDENCE AND VALIDATION
The QV600 chamber is currently being used by both academic and industrial labs worldwide, here’s a selection of the published papers that have used QV600 within their research.
A novel dual-flow bioreactor simulates increased fluorescein permeability in epithelial tissue barriers.
Authors: Giusti, S. et al., 2014.
Journal: Biotechnology Journal, 9, pp.1175–1184.
APPLICATIONS
Commercially available inserts can easily be fitted to the QV600 chambers, giving them great flexibility and a wide variety of applications:
Surface cultures
- Cornea
- Skin
- Respiratory System
Barrier models
- Gut model
- Blood-Brain Barrier
- Kidney
STARTER KIT
The QV600 starter kit contains everything you need to add flow to your experiments (except a peristaltic pump) – 3 chambers, tubing & reservoir bottle to form a complete circuit, holding trays for the complete system and a user guide.
QV600 MANUAL
Fill out the form below to download the QV600 Starter Kit User Manual.
Complete the below contact form to Download the QV600 ALI protocol
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Address: Kirkstall Ltd
3 Aspen Court
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