Cell Culture Chamber

The QV600 chamber has been designed for surface cultures, such as skin, respiratory epithelium or whole corneas. Additionally, the use of transwells to create a double cavity chamber is ideal for the study of membranes and barrier models.

Is the QV600 system applicable to your project? Contact us to find out!

The QV600 provides a constant medium surface, positioned to allow the membrane on commercial inserts to sit at this height and provide cell cultures with a continuous perfusion of media as well as exposure to a controlled gaseous environment.

The QV600 chamber can also be fitted with an alternative insert to create a double cavity chamber ideal for the study of membranes and barrier models.

KEY BENEFITS

Users of the QV600 can expect to profit from a number of key benefits associate with the QV600 cell culture chambers:

Continuous flow of medium across cell cultures
Consistent ALI level within chambers – no risk of cells drying due to evaporation
Ports for control of gas within culture
Simple fluid level control
Sized for commercially available inserts

SPECIFICATION

Chamber width	15 mm internal
Chamber depth	33 mm, minimum depth 12 mm
Materials	Chamber: PDMS Tubing: Tygon Luers and reservoir bottle: Polypropylene
Overall dimensions	23 mm height x 37 mm diameter
Diameter of tubing	Inlet: 1/16” ID Outlet: 3/32” ID
Volume of chamber	2 ml running as ALI, 2 x 2 ml with barrier insert

QV600 EVIDENCE AND VALIDATION

The QV600 chamber is currently being used by both academic and industrial labs worldwide, here’s a selection of the published papers that have used QV600 within their research.

A novel dual-flow bioreactor simulates increased fluorescein permeability in epithelial tissue barriers.

Authors: Giusti, S. et al., 2014.
Journal: Biotechnology Journal, 9, pp.1175–1184.

VIEW PAPER

APPLICATIONS

Commercially available inserts can easily be fitted to the QV600 chambers, giving them great flexibility and a wide variety of applications:

Surface cultures

Cornea
Skin
Respiratory System

Barrier models

Gut model
Blood-Brain Barrier
Kidney

STARTER KIT

The QV600 starter kit contains everything you need to add flow to your experiments (except a peristaltic pump) – 3 chambers, tubing & reservoir bottle to form a complete circuit, holding trays for the complete system and a user guide.

GET STARTED