Cell Culture Chamber
The QV500 cell culture chamber is a highly flexible research tool, ideal for academic use. Produced from medical-grade silicone, the chamber provides a leak proof seal, allowing continuous flow of media across and through cell cultures.
Is the QV500 system applicable to your project? Contact us to find out!
The chamber has a common diameter with the wells in standard 24-well plates, allowing for protocols developed for static cultures to easily be transferred to the system, as well as providing compatibility with both coverslips and a range of 3D scaffolds.
The modular design allows the system to be customised to provide the most suitable model for each specific experiment.
Users of the QV500 can expect to profit from a number of key benefits associated with the QV500 cell culture chambers:
- Continuous replenishment of media
- Recirculation of media allows self-conditioning by cells
- Prevents necrosis in 3D culture
- Modular, flexible system
- Standard well size
- More physiological relevance
- Reservoir allows easier media change without shock to culture
- Sealed system design ensures sterility in operation
- Repeat usage
|Chamber width||15 mm internal|
|Chamber depth||10 mm from culture surface to top of chamber base|
Luers and reservoir bottle: Polypropylene
|Overall dimensions||23 mm height x 37 mm diameter|
|Diameter of tubing||Inlet: 1/16” ID
Outlet: 3/32” ID
|Volume of chamber||2 ml|
QV500 EVIDENCE AND VALIDATION
The QV500 chamber is currently being used by both academic and industrial laboratories worldwide, here’s a selection of the published papers that have used QV500 within their research.
Glucose and fatty acid metabolism in a 3 tissue in-vitro model challenged with normo- and hyperglycaemia
Authors: Iori, E. et al., 2012.
Journal: PLoS ONE, 7(4), pp.1–9.
Design Criteria for Generating Physiologically Relevant In Vitro Models in Bioreactors.
Authors: Mattei, G., Giusti, S. & Ahluwalia, A., 2014
Journal: Processes, 2, pp.548–569.
In vitro generation of functional liver organoid-like structures using adult human cells.
Authors: Ramachandran, S.D. et al., 2015.
Journal: PLoS ONE, 10(10), pp.1–14.
The QV500 is designed to eliminate bubbles and provide laminar flow. It allows for submerged cell culture, whilst the modular nature allows for interconnecting cell co-culture:
- Long-Term Exposure
- Repeat dosage
- Systemic system models & interactions
The QV500 starter kit contains all of the parts you need to set up a Quasi Vivo® system. With the addition of a peristaltic pump, the kit provides everything you need to convert your existing 24-well static cultures to flow. In addition to three chambers and a reservoir bottle, the kit also contains ample tubing and connectors to set up the system, as well as holding trays for the complete system and a comprehensive user guide.
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Kirkstall Ltd 3
Centurion Business Park
Rotherham S60 1FB
+ 44 (0) 1709 361 241